H2N2V1, Main, Exploration, bibRecord, 002A43

Comparative studies of wild-type and 'cold-mutant' (temperature sensitive) influenza viruses: geneology of the matrix (M) and non-structural (NS) proteins in recombinant cold-adapted H3N2 viruses.

Identifieur interne : 002A43 ( Main/Exploration ); précédent : 002A42; suivant : 002A44

Comparative studies of wild-type and 'cold-mutant' (temperature sensitive) influenza viruses: geneology of the matrix (M) and non-structural (NS) proteins in recombinant cold-adapted H3N2 viruses.

Auteurs : A P Kendal ; N J Cox ; B R Murphy ; S B Spring ; H F Maassab

Source :

The Journal of general virology [ 0022-1317 ] ; 1977.

RBID : pubmed:915481

Descripteurs français

KwdFr :
- ARN viral (analyse), Adaptation biologique, Basse température, Gènes viraux, Mutation, Peptides (analyse), Protéines virales (analyse), Protéines virales (génétique), Recombinaison génétique, Sous-type H3N2 du virus de la grippe A, Virus de la grippe A (analyse), Virus de la grippe A (croissance et développement), Virus de la grippe A (génétique).
MESH :
- analyse : ARN viral, Peptides, Protéines virales, Virus de la grippe A.
- croissance et développement : Virus de la grippe A.
- génétique : Protéines virales, Virus de la grippe A.
- Adaptation biologique, Basse température, Gènes viraux, Mutation, Recombinaison génétique, Sous-type H3N2 du virus de la grippe A.

English descriptors

KwdEn :
- Adaptation, Biological, Cold Temperature, Genes, Viral, Influenza A Virus, H3N2 Subtype, Influenza A virus (analysis), Influenza A virus (genetics), Influenza A virus (growth & development), Mutation, Peptides (analysis), RNA, Viral (analysis), Recombination, Genetic, Viral Proteins (analysis), Viral Proteins (genetics).
MESH :
- chemical , analysis : Peptides, RNA, Viral, Viral Proteins.
- analysis : Influenza A virus.
- genetics : Influenza A virus, Viral Proteins.
- growth & development : Influenza A virus.
- Adaptation, Biological, Cold Temperature, Genes, Viral, Influenza A Virus, H3N2 Subtype, Mutation, Recombination, Genetic.

Abstract

The matrix (M) protein of the H2N2 virus A/Ann Arbor/6/60 may be distinguished from M protein of several H3N2 viruses and A/New Jersey/76 (HSWINI) by SDS acrylamide gel electrophoresis using a discontinuous buffer system. The smallest RNA (RNA 8) of the A/Ann Arbor/6/60 virus may be distinguished from RNA 8 of several H3N2 viruses by acrylamide gel electrophoresis in 3% or 3-6% gels in the absence of urea, if electrophoresis is done at 30 to 36 degrees C or 20 degrees C respectively. Ten clones of conditionally-lethal temperature-sensitive (ts) mutants were studied, which derived their cold-adaption and ts genes from mutant A/Ann Arbor/6/60, and their haemagglutinin from the H3N2 virus A/Scotland/840/74. Each clone was found to derive its M protein from A/Ann Arbor/6/60 mutant, and its RNA 8 from A/Scotland/840/74. The only assignment of genes 7 and 8 consistent with these findings for the recombinants is that in each parent virus (and in the recombinants) gene 7 codes for M protein, and gene 8 for NS protein. Furthermore, it may be concluded from the results that the biologically important ts lesions in the A/Ann Arbor/6/60 mutant parent are not present in the NS gene. In addition to the recombinants of A/Ann Arbor/6/60 and A/Scotland/840/74, five independent ts/cold-adapted recombinants of A/Ann Arbor/6/60 mutant with H3N2 and HSWINI wild-type viruses were examined, and all were found to contain the M protein of the A/Ann Arbor/6/60 mutant parent. This is suggestive that M protein may be at least partially responsible for the cold-adaptation and/or ts properties of the A/Ann Arbor/6/60 mutant and the recombinants.

DOI: 10.1099/0022-1317-37-1-145
PubMed: 915481

Affiliations:

Le document en format XML

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<term>Influenza A virus (analysis)</term>
<term>Influenza A virus (genetics)</term>
<term>Influenza A virus (growth & development)</term>
<term>Mutation</term>
<term>Peptides (analysis)</term>
<term>RNA, Viral (analysis)</term>
<term>Recombination, Genetic</term>
<term>Viral Proteins (analysis)</term>
<term>Viral Proteins (genetics)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term>
<term>Adaptation biologique</term>
<term>Basse température</term>
<term>Gènes viraux</term>
<term>Mutation</term>
<term>Peptides (analyse)</term>
<term>Protéines virales (analyse)</term>
<term>Protéines virales (génétique)</term>
<term>Recombinaison génétique</term>
<term>Sous-type H3N2 du virus de la grippe A</term>
<term>Virus de la grippe A (analyse)</term>
<term>Virus de la grippe A (croissance et développement)</term>
<term>Virus de la grippe A (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Peptides</term>
<term>RNA, Viral</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term>
<term>Peptides</term>
<term>Protéines virales</term>
<term>Virus de la grippe A</term>
</keywords>
<keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Influenza A virus</term>
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<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus de la grippe A</term>
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<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term>
<term>Viral Proteins</term>
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<term>Cold Temperature</term>
<term>Genes, Viral</term>
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<term>Mutation</term>
<term>Recombination, Genetic</term>
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<term>Basse température</term>
<term>Gènes viraux</term>
<term>Mutation</term>
<term>Recombinaison génétique</term>
<term>Sous-type H3N2 du virus de la grippe A</term>
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<front><div type="abstract" xml:lang="en">The matrix (M) protein of the H2N2 virus A/Ann Arbor/6/60 may be distinguished from M protein of several H3N2 viruses and A/New Jersey/76 (HSWINI) by SDS acrylamide gel electrophoresis using a discontinuous buffer system. The smallest RNA (RNA 8) of the A/Ann Arbor/6/60 virus may be distinguished from RNA 8 of several H3N2 viruses by acrylamide gel electrophoresis in 3% or 3-6% gels in the absence of urea, if electrophoresis is done at 30 to 36 degrees C or 20 degrees C respectively. Ten clones of conditionally-lethal temperature-sensitive (ts) mutants were studied, which derived their cold-adaption and ts genes from mutant A/Ann Arbor/6/60, and their haemagglutinin from the H3N2 virus A/Scotland/840/74. Each clone was found to derive its M protein from A/Ann Arbor/6/60 mutant, and its RNA 8 from A/Scotland/840/74. The only assignment of genes 7 and 8 consistent with these findings for the recombinants is that in each parent virus (and in the recombinants) gene 7 codes for M protein, and gene 8 for NS protein. Furthermore, it may be concluded from the results that the biologically important ts lesions in the A/Ann Arbor/6/60 mutant parent are not present in the NS gene. In addition to the recombinants of A/Ann Arbor/6/60 and A/Scotland/840/74, five independent ts/cold-adapted recombinants of A/Ann Arbor/6/60 mutant with H3N2 and HSWINI wild-type viruses were examined, and all were found to contain the M protein of the A/Ann Arbor/6/60 mutant parent. This is suggestive that M protein may be at least partially responsible for the cold-adaptation and/or ts properties of the A/Ann Arbor/6/60 mutant and the recombinants.</div>
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Comparative studies of wild-type and 'cold-mutant' (temperature sensitive) influenza viruses: geneology of the matrix (M) and non-structural (NS) proteins in recombinant cold-adapted H3N2 viruses.

Comparative studies of wild-type and 'cold-mutant' (temperature sensitive) influenza viruses: geneology of the matrix (M) and non-structural (NS) proteins in recombinant cold-adapted H3N2 viruses.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

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